Journal: JBMR Plus
Article Title: Multi-tissue spatial transcriptomics identified simultaneous responses to oxidative stress and apoptosis in parallel with tissue-specific reprogramming in modeled chronic kidney disease-mineral and bone disorder
doi: 10.1093/jbmrpl/ziaf161
Figure Lengend Snippet: Spatial transcriptomics identified tissue subtypes in muscle and bone. (A-C) Plasma intact FGF23 and serum TNF and blood urea nitrogen (BUN) levels in control (Ctrl) and CKD-MBD (CKD) mice (* p < .05; ** p < .01; *** p < .001 vs controls). H&E bone-muscle cross-sections for Ctrl (D) and CKD (E) and the Seurat clusters mapped on the sections (F and G). (H) Tissue and fiber type specific marker gene expression. (I) UMAP of Ctrl and CKD samples, with major tissue and muscle fiber types labeled. (J) Localization of major muscle fiber types in Ctrl and CKD tissue sections (colors correspond to UMAP in H).
Article Snippet: Serum TNFα was measured using a mouse specific Quantikine ELISA for TNFα (R&D Systems).
Techniques: Clinical Proteomics, Control, Marker, Gene Expression, Labeling